Durchflusszytometrie

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The facility provides a centralized flow cytometry and cell sorting service to researchers and collaborators at the Max Planck Institute for Heart and Lung Research. We provide serivce to a wide range of applications that include samples from murine tissues, zebrafish, and various cell lines for gene expression studies, cell cycle analysis and multi-colour phenotyping.

Cell Sorters

Bigfoot                                                         
Bigfoot is a high end cell sorter equipped with 5 lasers (340nm, 405nm, 488nm, 561nm, and 640nm)  and 55 detectors which allows for both conventional or spectral cell sorting. It is a jet-in-air sorter that can sort straight down or multi stream into 96 well or 384 well plate, simultaneous 6-way bulk sorting into different sized collection tubes, or sorting into 10x Genomics plate.                                                        
                                                        
Aria III                                                        
Aria III cell sorter is a high end cell sorter equipped with 4-lasers (405nm, 488nm, 561nm and 640nm)  and 12 fluorescence emission channels. It is a cuvette based sorter that can sort upto 4 populations at the same time, and into a 96-well or 384-well plate.                                                        
                                                        
BioSorter                                                        
The BioSorter is a large particle sorter equipped with 3 lasers ( 405nm, 488nm, and 561 nm)  and comes with different flow cell sizes of 250mM, 500mM, and 1000mM. This allows for sorting a range of large sized entities e.g. cardiomyocytes, mouse pancreatic islets, adipocytes or whole organisms such as C.elegans, and zebrafish larvae.                                                        
                      
S3e                                                        
The S3e is a compact sorter that is equipped with 2 lasers (488nm, and 561 nm) and 4 fluorescence emission channels. It can sort 2-way into different sized collection tubes.                                                        
                       
MACS-Classic sorter                                                        
This magnetic bead based sorter is used for the pre-enrichment of rare populations.                                                       

Analyzers

LSR Fortessa                                                        
The LSR Fortessa - SORP is equipped with 5 lasers ( 355nm, 405nm, 488nm, 561nm, and 640nm) and 18 fluorescence emission channels. All analysis experiments from cell cycle analysis to multiparametric phenotyping are performed on this instrument.