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Durchflusszytometrie

Servicegruppe

Diese Informationen sind derzeit nur in englischer Sprache verfügbar.

 

Group leader/Facility manager:

Ann Atzberger

 

Group members:
Kerstin Richter
Khrievono Kikhi

The Flow Cytometry Facility

The facility provides a centralized flow cytometry and cell sorting service to researchers and collaborators at the Max Planck Institute for Heart and Lung Research. It covers a wide range of applications that include mouse satellite cells, lung endothelial cells, cardiomyocytes, and diverse samples for gene expression, cell cycle and multi-colour phenotyping.

Cell sorters

 

BioSorter

The BioSorter is a large particle sorter equipped with all options for isolating a range of different sized entities e.g. mouse pancreatic islets, adipocytes, myofibers and zebrafish.

FACSAriaII1 sorter

This state-of-the-art cell sorter has 4-lasers and 11 fluorescence emission channels.

 

JSAN Cell sorter

These instruments contain a 488nm-laser with 4 fluorescence emission channels.

 

DEPArray sorter

The DEPArray uses a six-channel fluorescent microscope and CCD camera to capture images and identify cells. Dielectrophoresis (DEP) is used to capture individual cells in “DEP cages” that are then moved into the holding chamber for isolation and recovery.

 

MACS-Classic sorter

This magnetic bead based sorter is used for the pre-enrichment of rare populations.

 

Analyzers

 

LSR Fortessa

5-lasers with 18 emission channels.

 

FacsCanto

3-Lasers with 9-emission channels.

Contact:

 

Ann Atzberger, Facility Manager

Tel.: +49 (0)6032 705-1774 (Office)

Tel.: +49 (0)6032 705-1701 (Lab)

 

Group members:

Kerstin Richter

Tel.: +49 (0)6032 705-1701

+49 6032 705-1160

Khrievono Kikhi

Tel.: +49 (0)6032 705-1701

© 2019 Max-Planck-Institut für Herz- und Lungenforschung, Bad Nauheim